In vivo conversion of labeled fatty acids to the sphingolipid fatty acids in rat brain*

In vivo conversion of labeled fatty acids to the sphingolipid fatty acids in rat brain*

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Emulsions of carboxyl-labeled palmitate, stearate, lignocerate, and dl-cerebronate were injected into the brains of weanling rats.After various time intervals, the rats were sacrificed, and the saturated fatty acids of the brain sphingolipids were isolated and decarboxylated.All four acids had been incorporated into the sphingolipids.

The palmitate and stearate gave rise to radioactive lignocerate and cerebronate in which the carboxyl groups were relatively nonradioactive, Acteoside and ursolic acid synergistically protects H2O2-induced neurotrosis by regulation of AKT/mTOR signalling: from network pharmacology to experimental validation indicating the latter acids are made by chain lengthening of Organization of Accountance in Health and Fitness Institutions the shorter acids.The lignocerate-C14 gave rise to labeled cerebronate in which the carboxyl group contained nearly all the activity, showing that the normal acid is converted fairly directly into the hydroxy acid.Some radioactivity was found also in the shorter acids, apparently by the intermediate degradation of lignocerate to acetate.

The injected cerebronate-C14 underwent little or no conversion to normal acids.